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1% Triton X-100, and 1&215; protease inhibitor cocktail) and once more with 50mM TRIS-HCl, pH 7. &0183;&32;Gels were transferred to nitrocellulose membranes (Biorad) using the Bio-Rad semidry transfer and blocked for 1 hour in filtered 5% BSA. Relative luminescence is shown as mean values with S.
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